Chromatography A Laboratory Technique - 1629 Words

Introduction Chromatography, which was originally discovered and developed by Mikhail Semenovich Tswett in the early 1900s, is a laboratory technique used to separate and distinguish between components of a mixture. The various components of a sample mixture are known as analytes. Chromatography was originally used to separate the various molecules that make up plant pigmentation. Because these components, carotenes, chlorophyll, and xanthophylls, are different colors, the separation of them is responsible for the name of this laboratory technique. Throughout the early to mid-1900s, different styles of chromatography were developed and allowed this technique to be applicable to a wide variety of analyses and separation processes. Chromatography can be applied to analytical process, which aim to examine the presence and relative concentrations of various analytes within a sample. The goal of preparative chromatography is to separate analytes within a mixture for isolation and purifica tion purposes. Chromatography was originally developed by Tswett for the purpose of isolation of particular compounds (10). Moreover, more recent advancements in chromatography are facilitating the separation and distinction of analytes that are extremely comparable to one another and increasing the resolution of the technique. Gas chromatography (GC) is a chromatography technique that was developed around the mid-1900s. In addition to being the first among chromatography equipment to beShow MoreRelatedDetection or Identification of Trace Amounts of Explosives1624 Words   |  6 PagesThe detection and/or identification of trace amounts of explosives. The review of each method should include a description of the way the method works and the advantages and drawbacks of the technique. Introduction An explosive, are reactive substances that contain a large amount of potential energy that can yield an explosion if they are released rapidly, they are accompanied by the production of light, heat, sound, and pressure. Mainly used in the mining industry for extraction of mineralsRead MoreInvestigating The Substances Of Water Chromatography1350 Words   |  6 Pageswhich toxic ions might be present in a sample. Chromatography is one of the first tools used in such situations. In this technique, many types of mixtures can be separated into the component pure substances; by comparison to a standard sample, each component substance can also be tentatively identified. Many varieties of chromatography exist, each one designed to separate specific types of mixtures. The common feature of each type of chromatography is that a mobile phase (a liquid or gas) is pushedRead MoreTesting Of Drugs And Its Effect On Society711 Words   |  3 Pagesare made in laboratories by people who are unsk illed and untrained. Urine There are two steps for testing of drugs. One is screening and the other is confirmatory test. Screening test is used to find the presence of drug or to identify a specific drug. The screening test for drugs are cheap and less time consuming. But the drawback of this test is not precise as confirmatory test. For detection of drugs in urine, there is two screening methods such as immunoassay and chromatography. Urine samplesRead MoreSeparation of Chlorophyll a, Chlorophyll B, and Beta Carotene by Paper Chromatography1323 Words   |  6 PagesAbstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so itRead MoreFormal Report on Exp 51412 Words   |  6 PagesCOLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana, Mariah Ericka M. Patawaran, Princess Juneire M. Peligro, Francisco Q. Pua III, Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography, as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the coloredRead MoreThe Effects Of Color Additives On Food Color1561 Words   |  7 Pagesloops over, bland, naturally colored cereal. Chromatography is the term for the laboratory techniques used in the separation of mixtures. The mixture is dissolved in a fluid, which is called the mobile phase. The mobile phase carries the mixture through a structure holding another material, which is called the stationary phase. The various components of the mixture travel at different speeds, which causes them to separate. Chromatography is technique generally used for chemical analysis in scientificRead MoreThin Layer Chromatography Used For Separate Non Volatile Mixtures846 Words   |  4 PagesThin Layer Chromatography I. Introduction Column chromatography is a technique used to separate non-volatile mixtures. A sub-class to column chromatography is thin layer chromatography. Thin layer Chromatography is usually being used to separate compounds by the distribution between two phases. The two phases are mobile and stationary phase. It is usually performed on a sheet of glass coated with a thin layer of adsorbent silica named TLC plate. This layer of adsorbent is known as the stationaryRead MoreEssay Chromatography Investigation933 Words   |  4 PagesChromatography Investigation Chromatography is a highly regarded technique used to separate the components of a mixture. It is based on the principle that each component possesses a unique affinity for a stationary phase and a mobile phase. The components that are more inclined to enter the mobile phase will migrate further on the chromatogram and distinguish themselves from the other components. The type of solvent used in chromatography is known to directly affectRead MoreLab Report On Methylcyclohexene1541 Words   |  7 PagesMethylcyclohexene Ashton Krstevski Lab Partner: Micheal O’Daniel Organic Chemistry 1 Laboratory, Indiana University Northwest, Gary, Indiana 46408 October 28, 2016 Abstract: The purpose of this experiment was to practice the functional group transformation procedure. The process of the experiment included the dehydration of 2-methylcyclohexanol in the presence of phosphoric acid and heat. The products that were formed from the reaction were 1-methylcyclohexene and 3-methylcyclohexene. The mass ofRead MoreChromatography1984 Words   |  8 PagesCOLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos, Kuia B. Allarde, Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment, the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to